Monoclonal Anti-NPHS1 antibody produced in mouse
SIGMA/SAB1402860 - clone 3H6, purified immunoglobulin, buffered aqueous solution
Synonym: CNF; NPHN
Product Type: Chemical
Immunoblotting Monoclonal Anti-NPHS1: Cat. No. SAB1402860: Antibody concentration: 1 μg/mL. Specific band of ~36 kDa using immunogen protein lysate.
Western Blotting QC Western Blot detection against Immunogen (35.64 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3H6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~36.01 kDa |
| NCBI accession no. | NM_004646   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O60500 |
| General description: | Nephrin is a kidney glomerular filtration barrier protein that is an essential component of the interpodocyte-spanning slit diaphragm. Mutations in the nephrin gene are associated with congenital nephrotic syndrome (NPHS1; MIM 256300).[supplied by OMIM |
| Immunogen: | NPHS1 (NP_004637.1, 33 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence GFWALPENLTVVEGASVELRCGVST |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |