Monoclonal Anti-PDYN, (C-terminal) antibody produced in mouse
SIGMA/SAB1402867 - clone 2E12, purified immunoglobulin, buffered aqueous solution
Synonym: MGC26418; PENKB
Product Type: Chemical
Western Blotting Western Blot analysis of PDYN expression in transfected 293T cell line by PDYN monoclonal antibody, clone 2E12. Lanes Lane 1: PDYN transfected lysate (28.4 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (31.24 kDa).
Immunoprecipitation Immunoprecipitation of PDYN transfected lysate using anti-PDYN monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PDYN rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged PDYN is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E12, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~31.61 kDa |
| NCBI accession no. | NM_024411   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P01213 |
| General description: | The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. (provided by RefSeq) |
| Immunogen: | PDYN (NP_077722.1, 205 a.a. ~ 254 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KRYGGFLRRIRPKLKWDNQKRYGGF |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |