Monoclonal Anti-POU4F1 antibody produced in mouse
SIGMA/SAB1402874 - clone 7B4, purified immunoglobulin, buffered aqueous solution
Synonym: Mouse Anti-POU4F1; POU4F1 Detection Antibody
Product Type: Chemical
Western Blotting Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody, clone 7B4. Lanes Lane 1: POU4F1 transfected lysate (46.09 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (35.64 kDa).
ELISA Detection limit for recombinant GST tagged POU4F1 is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 7B4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~36.01 kDa |
| NCBI accession no. | NM_006237   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q01851 |
| General description: | BRN3A (POU4F1) is a class IV POU domain-containing transcription factor highly expressed in the developing sensory nervous system and in cells of the B- and T-lymphocytic lineages (Gerrero et al., 1993 [PubMed 8248179]).[supplied by OMIM |
| Immunogen: | POU4F1 (NP_006228.2, 331 a.a. ~ 420 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QAWLEEAEGAQREKMNKPELFNGGE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |