Monoclonal Anti-RFC5 antibody produced in mouse
SIGMA/SAB1402894 - clone 1B1, purified immunoglobulin, buffered aqueous solution
Synonym: MGC1155; RFC36
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to RFC5 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting RFC5 monoclonal antibody, clone 1B1. Western Blot analysis of RFC5 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (63.14 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~63.51 kDa |
| NCBI accession no. | BC013961   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P40937 |
| General description: | The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported. (provided by RefSeq) |
| Immunogen: | RFC5 (AAH13961, 1 a.a. ~ 340 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence METSALKQQEQPAATKIRNLPWVEK |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |