Monoclonal Anti-FZD4 antibody produced in mouse
SIGMA/SAB1402984 - clone 3G7, purified immunoglobulin, buffered aqueous solution
Synonym: CD344; EVR1; FEVR; FZD4S; Fz-4; FzE4; GPCR; MGC34390
Product Type: Chemical
Western Blotting Western Blot analysis of FZD4 expression in transfected 293T cell line by FZD4 monoclonal antibody, clone 3G7. Lanes Lane 1: FZD4 transfected lysate (Predicted MW: 59.9 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged FZD4 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~37.11 kDa |
| NCBI accession no. | NM_012193   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9ULV1 |
| General description: | This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. (provided by RefSeq) |
| Immunogen: | FZD4 (NP_036325, 107 a.a. ~ 206 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TEKINIPIGPCGGMCLSVKRRCEPV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |