Monoclonal Anti-FCN3 antibody produced in mouse
SIGMA/SAB1402990 - clone 4B4, ascites fluid
Synonym: FCNH; HAKA1; MGC22543
Product Type: Chemical
Western Blotting Western Blot analysis of FCN3 expression in transfected 293T cell line by FCN3 monoclonal antibody, clone 4B4. Lanes Lane 1: FCN3 transfected lysate (31.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (57.42 kDa).
Immunoprecipitation Immunoprecipitation of FCN3 transfected lysate using anti-FCN3 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with FCN3 rabbit polyclonal antibody.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4B4, monoclonal |
| conjugate | unconjugated |
| isotype | IgMκ |
| mol wt | antigen ~57.79 kDa |
| NCBI accession no. | BC020731   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O75636 |
| General description: | Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified. (provided by RefSeq) |
| Immunogen: | FCN3 (AAH20731, 1 a.a. ~ 288 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDLLWILPSLWLLLLGGPACLKTQE |
| Physical form: | Clear solution |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |