Monoclonal Anti-PUF60 antibody produced in mouse
SIGMA/SAB1403114 - clone 1C1, purified immunoglobulin, buffered aqueous solution
Synonym: FIR; FLJ31379; RoBPI; SIAHBP1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PUF60 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody, clone 1C1. Lanes Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 kDa). Lane 2: Non-transfected lysate.
ELISA Detection limit for recombinant GST tagged PUF60 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~82.87 kDa |
| NCBI accession no. | BC011979   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9UHX1 |
| General description: | The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. (provided by RefSeq) |
| Immunogen: | PUF60 (AAH11979, 1 a.a. ~ 516 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MENGQSTAAKLGLPPLTPEQQEALQ |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |