Monoclonal Anti-APTX antibody produced in mouse
SIGMA/SAB1403275 - clone 2H6, purified immunoglobulin, buffered aqueous solution
Synonym: AOA; AOA1; AXA1; EAOH; EOAHA; FHA-HIT; FLJ20157; MGC1072
Product Type: Chemical
Western Blotting Western Blot analysis of APTX expression in transfected 293T cell line by APTX monoclonal antibody, clone 2H6. Lanes Lane 1: APTX transfected lysate (Predicted MW: 29.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.52 kDa).
ELISA Detection limit for recombinant GST tagged APTX is 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2H6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aλ |
| mol wt | antigen ~36.89 kDa |
| NCBI accession no. | NM_175071   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q7Z2E3 |
| General description: | This gene encodes a member of the histidine triad (HIT) superfamily, some of which have nucleotide-binding and diadenosine polyphosphate hydrolase activities. The encoded protein may play a role in single-stranded DNA repair. Mutations in this gene have been associated with ataxia-ocular apraxia. Multiple transcript variants encoding distinct isoforms have been identified for this gene, however, the full length nature of some variants has not been determined. (provided by RefSeq) |
| Immunogen: | APTX (NP_778241.1, 69 a.a. ~ 166 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KLRFRLGYHAIPSMSHVHLHVISQD |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |