Monoclonal Anti-TNRC6C antibody produced in mouse
SIGMA/SAB1403333 - clone 3G11, purified immunoglobulin, buffered aqueous solution
Synonym: FLJ20015; KIAA1582
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to TNRC6C on HeLa cell. [antibody concentration 10 μg/mL]
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3G11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~36.89 kDa |
| NCBI accession no. | NM_018996   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| UniProt accession no. | Q9HCJ0 |
| Application: | Monoclonal Anti-TNRC6C antibody produced mouse is suitable for indirect ELISA. |
| Biochem/physiol Actions: | TNRC6C (Trinucleotide repeat containing 6C) plays an important role in the microRNA repression during protein synthesis via miRNA pathway. The C-terminal RRM RNA-binding domain mediates the process of translational repression. Its GW-rich and Q-rich domain also suppress the expression for protein synthesis by binding to a reporter mRNA. In addition to translational repression, the members of the GW182 family, also participate in the deadenylation and decay of miRNA by interacting with argonaute proteins. |
| General description: | Mouse monoclonal antibody raised against a partial recombinant TNRC6C. |
| General description: | TNRC6C (Trinucleotide repeat containing 6C) is a member of GW182 (Gly-Trp) family consisting of a domain similar to Drosophila GW182 (also known as Gawky), a domain rich in GW or WG repeats followed by a glutamine (Q)-rich region at the N-proximal end, a central ubiquitin-associated (UBA) domain and an RNA-binding domain termed as RRM. |
| Immunogen: | TNRC6C (NP_061869, 181 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TDGPNNTNPMNSSPNPINAMQTNGL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |