Monoclonal Anti-RBP7, (C-terminal) antibody produced in mouse
SIGMA/SAB1403445 - clone 4F4, purified immunoglobulin, buffered aqueous solution
Synonym: CRBP4; CRBPIV; MGC70641
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to RBP7 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of RBP7 expression in transfected 293T cell line by RBP7 monoclonal antibody, clone 4F4. Lanes Lane 1: RBP7 transfected lysate (Predicted MW: 15.5 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged RBP7 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~37.11 kDa |
| NCBI accession no. | NM_052960   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q96R05 |
| General description: | Due to its chemical instability and low solubility in aqueous solution, vitamin A requires cellular retinol-binding proteins (CRBPs), such as RBP7, for stability, internalization, intercellular transfer, homeostasis, and metabolism.[supplied by OMIM |
| Immunogen: | RBP7 (NP_443192.1, 35 a.a. ~ 134 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence KLLKPQKVIEQNGDSFTIHTNSSLR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |