Monoclonal Anti-AHSG, (C-terminal) antibody produced in mouse
SIGMA/SAB1403536 - clone 1D8, purified immunoglobulin, buffered aqueous solution
Synonym: A2HS; AHS; FETUA; HSGA
Product Type: Chemical
Western Blotting Western Blot analysis of AHSG expression in transfected 293T cell line by AHSG monoclonal antibody, clone 1D8. Lanes Lane 1: AHSG transfected lysate (39.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (64.13 kDa).
Immunoprecipitation Immunoprecipitation of AHSG transfected lysate using anti-AHSG monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with AHSG rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged AHSG is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~64.5 kDa |
| NCBI accession no. | BC048198   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P02765 |
| General description: | Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. (provided by RefSeq) |
| Immunogen: | AHSG (AAH48198.1, 19 a.a. ~ 367 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence APHGPGLIYRQPNCDDPETEEAALV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |