Monoclonal Anti-MAPK14, (C-terminal) antibody produced in mouse
SIGMA/SAB1403701 - clone 3D5, ascites fluid
Product Type: Chemical
Western Blotting MAPK14 monoclonal antibody, clone 3D5 Western Blot analysis of MAPK14 expression in C32.
Western Blotting Western Blot analysis of MAPK14 expression in transfected 293T cell line by MAPK14 monoclonal antibody, clone 3D5. Lanes Lane 1: MAPK14 transfected lysate (41.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3D5, monoclonal |
| conjugate | unconjugated |
| isotype | IgG1κ |
| mol wt | antigen ~37.11 kDa |
| NCBI accession no. | BC031574   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q16539 |
| General description: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. (provided by RefSeq) |
| Immunogen: | MAPK14 (AAH31574, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence QSLTQMPKMNFANVFIGANPLAVDL |
| Physical form: | Clear solution |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |