Monoclonal Anti-FASN antibody produced in mouse
SIGMA/SAB1403807 - clone 3B3-1D6, purified immunoglobulin, buffered aqueous solution
Synonym: FAS; MGC14367; MGC15706; OA-519; SDR27X1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to FASN on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of FASN expression in transfected 293T cell line by FASN monoclonal antibody, clone 3B3-1D6. Lanes Lane 1: FASN transfected lysate (48.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting FASN monoclonal antibody, clone 3B3-1D6. Western Blot analysis of FASN expression in human pancreas.
Western Blotting QC Western Blot detection against Immunogen (74.03 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B3-1D6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~74.4 kDa |
| NCBI accession no. | BC007909   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P49327 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. (provided by RefSeq) |
| Immunogen: | FASN (AAH07909, 1 a.a. ~ 439 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSTNDTIVSGTLPQRMASCLEVLDL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |