Monoclonal Anti-FH, (C-terminal) antibody produced in mouse
SIGMA/SAB1403819 - clone 3E7, purified immunoglobulin, buffered aqueous solution
Synonym: HLRCC; LRCC; MCL; MCUL1
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to FH on formalin-fixed paraffin-embedded human seminoma. [antibody concentration 3 μg/mL]
Western Blotting FH monoclonal antibody, clone 3E7 Western Blot analysis of FH expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (78.32 kDa).
ELISA Detection limit for recombinant GST tagged FH is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~78.69 kDa |
| NCBI accession no. | BC003108   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P07954 |
| General description: | The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. (provided by RefSeq) |
| Immunogen: | FH (AAH03108, 33 a.a. ~ 510 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VPSFWPPNAARMASQNSFRIEYDTF |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |