Monoclonal Anti-GABPA antibody produced in mouse
SIGMA/SAB1403848 - clone 4B8-G6, purified immunoglobulin, buffered aqueous solution
Synonym: E4TF1-60; E4TF1A; NFT2; NRF2; NRF2A
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody, clone 4B8-G6. Lanes Lane 1: GABPA transfected lysate (51.3 kDa). Lane 2: Non-transfected lysate.
Western Blotting GABPA monoclonal antibody, clone 4B8-G6 Western Blot analysis of GABPA expression in K-562.
Western Blotting QC Western Blot detection against Immunogen (75.68 kDa).
ELISA Detection limit for recombinant GST tagged GABPA is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4B8-G6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~76.05 kDa |
| NCBI accession no. | BC035031   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q06546 |
| General description: | This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. (provided by RefSeq) |
| Immunogen: | GABPA (AAH35031, 1 a.a. ~ 454 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTKREAEELIEIEIDGTEKAECTEE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |