Monoclonal Anti-GLUL, (C-terminal) antibody produced in mouse
SIGMA/SAB1403872 - clone 3B6, purified immunoglobulin, buffered aqueous solution
Synonym: GLNS; GS; PIG43; PIG59
Product Type: Chemical
Western Blotting GLUL monoclonal antibody, clone 3B6. Western Blot analysis of GLUL expression in HepG2.
Western Blotting Western Blot analysis of GLUL expression in transfected 293T cell line by GLUL monoclonal antibody, clone 3B6. Lanes Lane 1: GLUL transfected lysate (42.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting GLUL monoclonal antibody, clone 3B6 Western Blot analysis of GLUL expression in Jurkat.
Western Blotting GLUL monoclonal antibody, clone 3B6. Western Blot analysis of GLUL expression in PC-12.
Western Blotting GLUL monoclonal antibody, clone 3B6. Western Blot analysis of GLUL expression in Raw 264.7.
Western Blotting QC Western Blot detection against Immunogen (36.74 kDa).
ELISA Detection limit for recombinant GST tagged GLUL is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~37.11 kDa |
| NCBI accession no. | NM_002065   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P15104 |
| General description: | Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM |
| Immunogen: | GLUL (NP_002056, 274 a.a. ~ 373 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence IEKLSKRHQYHIRAYDPKGGLDNAR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |