Monoclonal Anti-HMGB2 antibody produced in mouse
SIGMA/SAB1403926 - clone 3C7, purified immunoglobulin, buffered aqueous solution
Synonym: HMG2
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 μg/mL]
Western Blotting HMGB2 monoclonal antibody, clone 3C7 Western Blot analysis of HMGB2 expression in HeLa S3 NE.
Western Blotting Western Blot analysis of HMGB2 expression in transfected 293T cell line by HMGB2 monoclonal antibody, clone 3C7. Lanes Lane 1: HMGB2 transfected lysate (22 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (47.19 kDa).
ELISA Detection limit for recombinant GST tagged HMGB2 is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3C7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~47.56 kDa |
| NCBI accession no. | BC000903   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P26583 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. (provided by RefSeq) |
| Immunogen: | HMGB2 (AAH00903.2, 1 a.a. ~ 195 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MGKGDPNKPRGKMSSYAFFVQTCRE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |