Monoclonal Anti-HSPA1B antibody produced in mouse
SIGMA/SAB1403949 - clone 2D11, purified immunoglobulin, buffered aqueous solution
Synonym: FLJ54328; HSP70-1B; HSP70-2; HSPA1A
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between EGFR and HSPA1B. HeLa cells were stained with anti-EGFR rabbit purified polyclonal 1:1200 and anti-HSPA1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting HSPA1B monoclonal antibody, clone 2D11. Western Blot analysis of HSPA1B expression in PC-12.
Western Blotting HSPA1B monoclonal antibody, clone 2D11 Western Blot analysis of HSPA1B expression in HeLa S3 NE.
Western Blotting HSPA1B monoclonal antibody, clone 2D11. Western Blot analysis of HSPA1B expression in NIH/3T3.
Western Blotting QC Western Blot detection against Immunogen (34.65 kDa).
ELISA Detection limit for recombinant GST tagged HSPA1B is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~35.02 kDa |
| NCBI accession no. | NM_005345   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P0DMV9 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. (provided by RefSeq) |
| Immunogen: | HSPA1B (NP_005336, 531 a.a. ~ 611 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VQRERVSAKNALESYAFNMKSAVED |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |