Monoclonal Anti-IRAK1, (C-terminal) antibody produced in mouse
SIGMA/SAB1403986 - clone 3A9, purified immunoglobulin, buffered aqueous solution
Synonym: IRAK; pelle
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting IRAK1 monoclonal antibody, clone 3A9 Western Blot analysis of IRAK1 expression in A-431.
Western Blotting Western Blot analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody, clone 3A9. Lanes Lane 1: IRAK1 transfected lysate (68 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi. Blot probed with IRAK1 monoclonal antibody (M02) clone 3A9. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (43.45 kDa).
Immunoprecipitation Immunoprecipitation of IRAK1 transfected lysate using anti-IRAK1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with IRAK1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged IRAK1 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3A9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~44.04 kDa |
| NCBI accession no. | BC054000   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P51617 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | IRAK1 (AAH54000, 530 a.a. ~ 693 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SSTGRAHSGAAPWQPLAAPSGASAQ |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |