Monoclonal Anti-IRF4, (C-terminal) antibody produced in mouse
SIGMA/SAB1403990 - clone 2D1, purified immunoglobulin, buffered aqueous solution
Synonym: LSIRF; MUM1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to IRF4 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of IRF4 expression in transfected 293T cell line by IRF4 monoclonal antibody, clone 2D1. Lanes Lane 1: IRF4 transfected lysate (51.8 kDa). Lane 2: Non-transfected lysate.
Enhanced Validation-RNAi Western blot analysis of IRF4 over-expressed 293 cell line, cotransfected with IRF4 Validated Chimera RNAi. Blot probed with IRF4 monoclonal antibody, clone 2D1. GAPDH (36.1 kDa) used as specificity and loading control.
Western Blotting QC Western Blot detection against Immunogen (37.84 kDa).
ELISA Detection limit for recombinant GST tagged IRF4 is approximately 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2D1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~38.21 kDa |
| NCBI accession no. | NM_002460   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q15306 |
| Biochem/physiol Actions: | Interferon regulatory factor 4 (IRF4) plays a vital role in regulation of immunoglobulin class switch recombination and plasma cell differentiation. In addition, it is also implicated in maintaining homeostasis of both mature B and mature T lymphocytes. Mutation in the gene is associated with the risk of developing chronic lymphocytic leukemia (CLL). |
| General description: | Interferon regulatory factor 4 (IRF4), also known as multiple myeloma oncogene-1 (MUM1), is a transcription factor. It is encoded by the gene mapped to human chromosome 6p25.3. The protein is specifically expressed in lymphocytes. |
| Immunogen: | IRF4 (NP_002451, 342 a.a. ~ 451 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LCNDRPNKLERDQTCKLFDTQQFLS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |