Monoclonal Anti-KRT18 antibody produced in mouse
SIGMA/SAB1404012 - clone 1G8, purified immunoglobulin, buffered aqueous solution
Synonym: CYK18; K18
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to KRT18 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting KRT18 monoclonal antibody, clone 1G8 Western Blot analysis of KRT18 expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (72.82 kDa).
ELISA Detection limit for recombinant GST tagged KRT18 is approximately 3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~73.41 kDa |
| NCBI accession no. | BC020982   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P05783 |
| General description: | KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. (provided by RefSeq) |
| Immunogen: | KRT18 (AAH20982, 1 a.a. ~ 430 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSFTTRSTFSTNYRSLGSVQAPSYG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |