Monoclonal Anti-LMNB1 antibody produced in mouse
SIGMA/SAB1404027 - clone 4E4, purified immunoglobulin, buffered aqueous solution
Synonym: ADLD; LMN; LMN2; LMNB; MGC111419
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to LMNB1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to LMNB1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting LMNB1 monoclonal antibody, clone 4E4. Western Blot analysis of LMNB1 expression in NIH/3T3.
Western Blotting LMNB1 monoclonal antibody, clone 4E4. Western Blot analysis of LMNB1 expression in Raw 264.7.
Western Blotting LMNB1 monoclonal antibody, clone 4E4. Western Blot analysis of LMNB1 expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (34.43 kDa).
ELISA Detection limit for recombinant GST tagged LMNB1 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4E4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~34.8 kDa |
| NCBI accession no. | NM_005573   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P20700 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. (provided by RefSeq) |
| Immunogen: | LMNB1 (NP_005564.1, 107 a.a. ~ 186 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence LGKCKAEHDQLLLNYAKKESDLNGA |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |