Monoclonal Anti-MYOC antibody produced in mouse
SIGMA/SAB1404105 - clone 2B4, purified immunoglobulin, buffered aqueous solution
Synonym: GLC1A; GPOA; JOAG; JOAG1; TIGR; myocilin
Product Type: Chemical
Western Blotting Western Blot analysis of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody, clone 2B4. Lanes Lane 1: MYOC transfected lysate (57 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (81.18 kDa).
ELISA Detection limit for recombinant GST tagged MYOC is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2B4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~81.55 kDa |
| NCBI accession no. | BC029261   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q99972 |
| General description: | MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma. (provided by RefSeq) |
| Immunogen: | MYOC (AAH29261, 1 a.a. ~ 504 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MRFFCARCCSFGPEMPAVQLLLLAC |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |