Monoclonal Anti-PCNA antibody produced in mouse
SIGMA/SAB1404178 - clone S1, purified immunoglobulin, buffered aqueous solution
Synonym: MGC8367
Product Type: Chemical
Western Blotting PCNA monoclonal antibody, clone S1. Western Blot analysis of PCNA expression in NIH/3T3.
Western Blotting Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody, clone S1. Lanes Lane 1: PCNA transfected lysate (28.8 kDa). Lane 2: Non-transfected lysate.
Western Blotting PCNA monoclonal antibody, clone S1. Western Blot analysis of PCNA expression in PC-12.
Western Blotting PCNA monoclonal antibody, clone S1. Western Blot analysis of PCNA expression in Raw 264.7.
Western Blotting PCNA monoclonal antibody, clone S1 Western Blot analysis of PCNA expression in HeLa S3 NE.
Western Blotting QC Western Blot detection against Immunogen (54.45 kDa).
ELISA Detection limit for recombinant GST tagged PCNA is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | S1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~54.82 kDa |
| NCBI accession no. | BC000491   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P12004 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. (provided by RefSeq) |
| Immunogen: | PCNA (AAH00491, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MFEARLVQGSILKKVLEALKDLINE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |