Monoclonal Anti-PCTK1, (N-terminal) antibody produced in mouse
SIGMA/SAB1404180 - clone 2E5, purified immunoglobulin, buffered aqueous solution
Synonym: FLJ16665; PCTAIRE; PCTAIRE1; PCTGAIRE
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PCTK1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of PCTK1 expression in transfected 293T cell line by PCTK1 monoclonal antibody, clone 2E5. Lanes Lane 1: PCTK1 transfected lysate (Predicted MW: 55.7 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (34.21 kDa).
Immunoprecipitation Immunoprecipitation of PCTK1 transfected lysate using anti-PCTK1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PCTK1 rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2E5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~34.8 kDa |
| NCBI accession no. | BC001048   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells. This gene is thought to escape X inactivation. Alternative splicing results in two transcript variants encoding different isoforms. (provided by RefSeq) |
| Immunogen: | PCTK1 (AAH01048, 1 a.a. ~ 80 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MDRMKKIKRQLSMTLRGGRGIDKTN |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |