Monoclonal Anti-PSAP antibody produced in mouse
SIGMA/SAB1404262 - clone 2F6, purified immunoglobulin, buffered aqueous solution
Synonym: FLJ00245; GLBA; MGC110993; SAP1
Product Type: Chemical
Western Blotting Western Blot analysis of PSAP expression in transfected 293T cell line by PSAP monoclonal antibody, clone 2F6. Lanes Lane 1: PSAP transfected lysate (Predicted MW: 58.1 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (83.16 kDa).
ELISA Detection limit for recombinant GST tagged PSAP is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~83.75 kDa |
| NCBI accession no. | BC004275   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P07602 |
| General description: | This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. (provided by RefSeq) |
| Immunogen: | PSAP (AAH04275, 1 a.a. ~ 524 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MYALFLLASLLGAALAGPVLGLKEC |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |