Monoclonal Anti-PTEN antibody produced in mouse
SIGMA/SAB1404272 - clone 3E7, purified immunoglobulin, buffered aqueous solution
Synonym: 10q23del; BZS; MGC11227; MHAM; MMAC1; PTEN1; TEP1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to PTEN on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting PTEN monoclonal antibody, clone 3E7 Western Blot analysis of PTEN expression in C32.
Western Blotting QC Western Blot detection against Immunogen (35.9 kDa).
Immunoprecipitation Immunoprecipitation of PTEN transfected lysate using anti-PTEN monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PTEN purified rabbit polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3E7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~35.9 kDa |
| NCBI accession no. | NM_000314   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P60484 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4, |
| Immunogen: | PTEN (NP_000305.1, 2 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence TAIIKEIVSRNKRRYQEDGFDLDLT |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |