Monoclonal Anti-S100A9 antibody produced in mouse
SIGMA/SAB1404348 - clone 4G9, purified immunoglobulin, buffered aqueous solution
Synonym: 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14
Product Type: Chemical
Western Blotting S100A9 monoclonal antibody, clone 4G9. Western Blot analysis of S100A9 expression in human kidney.
Western Blotting QC Western Blot detection against Immunogen (38.28 kDa).
ELISA Detection limit for recombinant GST tagged S100A9 is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4G9, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~38.65 kDa |
| NCBI accession no. | BC047681   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P06702 |
| Application: | Monoclonal Anti-S100A9 antibody produced in mouse is suitable for capture ELISA, indirect ELISA and western blot applications. |
| Biochem/physiol Actions: | S100A9 (S100 calcium binding protein A9) has a pro-inflammatory action in acute and chronic inflammation. In addition to inflammatory action, it exhibits cytokine-like activities for the receptor activation of advanced glycation end products (RAGE) and Toll-like receptor 4-dependent signaling cascades. S100A9 have been reported for neutrophil chemotaxis. It stimulates shedding of L-selectin, up-regulates and activates Mac-1 which finally induces neutrophil adhesion to fibrinogen in vitro. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. (provided by RefSeq) |
| Immunogen: | S100A9 (AAH47681, 1 a.a. ~ 114 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTCKMSQLERNIETIINTFHQYSVK |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |