Monoclonal Anti-SPAST antibody produced in mouse
SIGMA/SAB1404401 - clone 2F5, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to SPAST on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ~ 10 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to SPAST on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting SPAST monoclonal antibody, clone 2F5. Western Blot analysis of SPAST expression in Jurkat.
Western Blotting QC Western Blot detection against Immunogen (37.29 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F5, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~37.66 kDa |
| NCBI accession no. | NM_014946   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9UBP0 |
| General description: | This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. (provided by RefSeq) |
| Immunogen: | SPAST (NP_055761, 200 a.a. ~ 304 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence PVLPFSKSQTDVYNDSTNLACRNGH |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |