Monoclonal Anti-SPI1, (N-terminal) antibody produced in mouse
SIGMA/SAB1404402 - clone 2G1, purified immunoglobulin, buffered aqueous solution
Synonym: OF; PU.1; SFPI1; SPI-1; SPI-A
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to SPI1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting SPI1 monoclonal antibody, clone 2G1. Western Blot analysis of SPI1 expression in Raw 264.7.
Western Blotting QC Western Blot detection against Immunogen (38.94 kDa).
ELISA Detection limit for recombinant GST tagged SPI1 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2G1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~39.31 kDa |
| NCBI accession no. | NM_003120   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P17947 |
| General description: | This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | SPI1 (NP_003111, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MEGFPLVPPPSEDLVPYDTDLYQRQ |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |