Monoclonal Anti-STAT3 antibody produced in mouse
SIGMA/SAB1404414 - clone 1D11-2A11, purified immunoglobulin, buffered aqueous solution
Synonym: APRF; FLJ20882; HIES; MGC16063
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between STAT1 and STAT3. HeLa cells were stained with anti-STAT1 rabbit purified polyclonal 1:1200 and anti-STAT3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting STAT3 monoclonal antibody, clone 1D11-2A11 Western Blot analysis of STAT3 expression in HeLa.
Western Blotting STAT3 monoclonal antibody, clone 1D11-2A11. Western Blot analysis of STAT3 expression in K-562.
Western Blotting QC Western Blot detection against Immunogen (110.11 kDa).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1D11-2A11, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2bκ |
| mol wt | antigen ~110.7 kDa |
| NCBI accession no. | BC000627   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P40763 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. (provided by RefSeq) |
| Immunogen: | STAT3 (AAH00627, 1 a.a. ~ 769 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MAQWNQLQQLDTRYLEQLHQLYSDS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |