Monoclonal Anti-STK4 antibody produced in mouse
SIGMA/SAB1404424 - clone 4F4, purified immunoglobulin, buffered aqueous solution
Synonym: DKFZp686A2068; KRS2; MST1; YSK3
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to STK4 on HeLa cell. [antibody concentration 25 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between TP53 and STK4. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-STK4 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting STK4 monoclonal antibody, clone 4F4. Western Blot analysis of STK4 expression in PC-12.
Western Blotting STK4 monoclonal antibody, clone 4F4. Western Blot analysis of STK4 expression in Raw 264.7.
Western Blotting STK4 monoclonal antibody, clone 4F4 Western Blot analysis of STK4 expression in HeLa.
Western Blotting STK4 monoclonal antibody, clone 4F4. Western Blot analysis of STK4 expression in NIH/3T3.
Western Blotting QC Western Blot detection against Immunogen (36.19 kDa).
ELISA Detection limit for recombinant GST tagged STK4 is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~36.56 kDa |
| NCBI accession no. | NM_006282   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| proximity ligation assay: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13043 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it′s possible that this protein induces the chromatin condensation observed in this process. (provided by RefSeq) |
| Immunogen: | STK4 (NP_006273, 391 a.a. ~ 485 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence AKPSFLEYFEQKEKENQINSFGKSV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |