Monoclonal Anti-STX1A antibody produced in mouse
SIGMA/SAB1404425 - clone 1B11-1A8, purified immunoglobulin, buffered aqueous solution
Synonym: HPC-1; STX1; p35-1
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to STX1A on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting STX1A monoclonal antibody, clone 1B11-1A8. Western Blot analysis of STX1A expression in PC-12.
Western Blotting STX1A monoclonal antibody, clone 1B11-1A8 Western Blot analysis of STX1A expression in IMR-32.
Western Blotting Western Blot analysis of STX1A expression in transfected 293T cell line by STX1A monoclonal antibody, clone 1B11-1A8. Lanes Lane 1: STX1A transfected lysate (29 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (53.35 kDa).
ELISA Detection limit for recombinant GST tagged STX1A is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1B11-1A8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~53.72 kDa |
| NCBI accession no. | BC000444   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q16623 |
| General description: | Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM |
| Immunogen: | STX1A (AAH03011.1, 1 a.a. ~ 251 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MKDRTQELRTAKDSDDDDDVAVTVD |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |