Monoclonal Anti-TCF4 antibody produced in mouse
SIGMA/SAB1404449 - clone 1G4, purified immunoglobulin, buffered aqueous solution
Synonym: E2-2; ITF2; MGC149723; MGC149724; PTHS; SEF2; SEF2-1; SEF2-1A; SEF2-1B
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting TCF4 monoclonal antibody, clone 1G4 Western Blot analysis of TCF4 expression in K-562.
Western Blotting QC Western Blot detection against Immunogen (65.89 kDa).
ELISA Detection limit for recombinant GST tagged TCF4 is approximately 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1G4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~66.26 kDa |
| NCBI accession no. | BC031056   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P15884 |
| Biochem/physiol Actions: | Transcription factor 4 (TCF4) is essential for regulating Wnt-signaling pathway. It is linked to schizophrenia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Transcription factor 4 (TCF4) belongs to the class A subfamily of basic helix loop helix (bHLH) transcriptional regulators, which is also called E proteins. TCF4 gene is located on human chromosome 18q21.2. TCF4 is expressed in early human embryo. |
| Immunogen: | TCF4 (AAH31056.1, 1 a.a. ~ 365 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MHHQQRMAALGTDKELSDLLDFSAM |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |