Monoclonal Anti-TRAF1 antibody produced in mouse
SIGMA/SAB1404490 - clone 1A6, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Western Blotting QC Western Blot detection against Immunogen (71.5 kDa).
Immunoprecipitation Immunoprecipitation of TRAF1 transfected lysate using anti-TRAF1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with TRAF1 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged TRAF1 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1A6, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~71.87 kDa |
| NCBI accession no. | BC024145   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q13077 |
| General description: | The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors. (provided by RefSeq) |
| Immunogen: | TRAF1 (AAH24145, 1 a.a. ~ 416 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MASSSGSSPRPAPDENEFPFGCPPT |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |