Monoclonal Anti-TXN antibody produced in mouse
SIGMA/SAB1404501 - clone 6C10, purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to TXN on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting TXN monoclonal antibody, clone 6C10 Western Blot analysis of TXN expression in HeLa.
Western Blotting QC Western Blot detection against Immunogen (37.29 kDa).
ELISA Detection limit for recombinant GST tagged TXN is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6C10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~37.66 kDa |
| NCBI accession no. | BC003377   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P10599 |
| General description: | Thioredoxin is a 12-kD oxidoreductase enzyme containing a dithiol-disulfide active site. It is ubiquitous and found in many organisms from plants and bacteria to mammals. Multiple in vitro substrates for thioredoxin have been identified, including ribonuclease, choriogonadotropins, coagulation factors, glucocorticoid receptor, and insulin. Reduction of insulin is classically used as an activity test.[supplied by OMIM |
| Immunogen: | TXN (AAH03377, 1 a.a. ~ 105 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MVKQIESKTAFQEALDAAGDKLVVV |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |