Monoclonal Anti-VIL1 antibody produced in mouse
SIGMA/SAB1404525 - clone 2F10, purified immunoglobulin, buffered aqueous solution
Synonym: D2S1471; VIL
Product Type: Chemical
Western Blotting Western Blot analysis of VIL1 expression in transfected 293T cell line by VIL1 monoclonal antibody, clone 2F10. Lanes Lane 1: VIL1 transfected lysate (46.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (71.83 kDa).
ELISA Detection limit for recombinant GST tagged VIL1 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~72.42 kDa |
| NCBI accession no. | BC017303   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P09327 |
| General description: | This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. (provided by RefSeq) |
| Immunogen: | VIL1 (AAH17303, 1 a.a. ~ 421 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MTKLSAQVKGSLNITTPGLQIWRIE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |