Monoclonal Anti-BAG3 antibody produced in mouse
SIGMA/SAB1404732 - clone 5A8, ascites fluid
Synonym: BAG-3; BIS; CAIR-1; MGC104307
Product Type: Chemical
Western Blotting Western Blot analysis of BAG3 expression in transfected 293T cell line by BAG3 monoclonal antibody, clone 5A8. Lanes Lane 1: BAG3 transfected lysate (Predicted MW: 61.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (88.99 kDa).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 5A8, monoclonal |
| conjugate | unconjugated |
| isotype | IgG2bκ |
| mol wt | antigen ~89.36 kDa |
| NCBI accession no. | BC014656   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O95817 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. (provided by RefSeq) |
| Immunogen: | BAG3 (AAH14656.1, 1 a.a. ~ 575 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSAATHSPMMQVASGNGDRDPLPPG |
| Physical form: | Clear solution |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |