Monoclonal Anti-CDC42BPB antibody produced in mouse
SIGMA/SAB1404739 - clone 2F4, purified immunoglobulin, buffered aqueous solution
Synonym: KIAA1124; MRCKB
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to CDC42BPB on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of monoclonal antibody to CDC42BPB on A-431 cell. [antibody concentration 10 μg/mL]
Western Blotting CDC42BPB monoclonal antibody, clone 2F4. Western Blot analysis of CDC42BPB expression in PC-12.
Western Blotting CDC42BPB monoclonal antibody, clone 2F4 Western Blot analysis of CDC42BPB expression in A-431.
Western Blotting QC Western Blot detection against Immunogen (36.63 kDa).
ELISA Detection limit for recombinant GST tagged CDC42BPB is approximately 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 2F4, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~37 kDa |
| NCBI accession no. | AF128625   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9Y5S2 |
| General description: | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. (provided by RefSeq) |
| Immunogen: | CDC42BPB (AAD37506, 1580 a.a. ~ 1679 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence SKMISNPTNFNHVAHMGPGDGMQVL |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |