Monoclonal Anti-GIT2 antibody produced in mouse
SIGMA/SAB1404758 - clone M1, purified immunoglobulin, buffered aqueous solution
Synonym: CAT-2; DKFZp686G01261; KIAA0148; MGC760
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to GIT2 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of GIT2 expression in transfected 293T cell line by GIT2 monoclonal antibody, clone M1. Lanes Lane 1: GIT2 transfected lysate (Predicted MW: 52.6 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (77.33 kDa).
Immunoprecipitation Immunoprecipitation of GIT2 transfected lysate using anti-GIT2 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GIT2 rabbit polyclonal antibody.
ELISA Detection limit for recombinant GST tagged GIT2 is 1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~77.92 kDa |
| NCBI accession no. | BC001379   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q14161 |
| General description: | This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding. (provided by RefSeq) |
| Immunogen: | GIT2 (AAH01379, 1 a.a. ~ 471 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence MSKRLRSSEVCADCSGPDPSWASVN |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |