Monoclonal Anti-MFN2, (C-terminal) antibody produced in mouse
SIGMA/SAB1404765 - clone 6A8, purified immunoglobulin, buffered aqueous solution
Synonym: CMT2A; CMT2A2; CPRP1; HSG; KIAA0214; MARF
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of monoclonal antibody to MFN2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 μg/mL]
Western Blotting MFN2 monoclonal antibody, clone 6A8. Western Blot analysis of MFN2 expression in PC-12.
Western Blotting MFN2 monoclonal antibody, clone 6A8. Western Blot analysis of MFN2 expression in HeLa.
Western Blotting MFN2 monoclonal antibody, clone 6A8. Western Blot analysis of MFN2 expression in NIH/3T3.
ELISA Detection limit for recombinant GST tagged MFN2 is 0.3 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 6A8, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~36.78 kDa |
| NCBI accession no. | NM_014874   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | O95140 |
| General description: | This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified. (provided by RefSeq) |
| Immunogen: | MFN2 (NP_055689, 661 a.a. ~ 757 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence FKRQFVEHASEKLQLVISYTGSNCS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |