Monoclonal Anti-DIDO1 antibody produced in mouse
SIGMA/SAB1404898 - clone 3B1, purified immunoglobulin, buffered aqueous solution
Synonym: BYE1; C20orf158; DATF1; DIDO2; DIDO3; DIO-1; DIO1; DKFZp434P1115; FLJ11265
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to DIDO1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting DIDO1 monoclonal antibody, clone 3B1. Western Blot analysis of DIDO1 expression in HepG2.
Western Blotting QC Western Blot detection against Immunogen (36.41 kDa).
ELISA Detection limit for recombinant GST tagged DIDO1 is 0.03 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 3B1, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~37 kDa |
| NCBI accession no. | BC014489   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| immunofluorescence: suitable | |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | Q9BTC0 |
| General description: | Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms. (provided by RefSeq) |
| Immunogen: | DIDO1 (AAH14489, 321 a.a. ~ 420 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence ILQVQDETHSETADQQEAKWRPGDA |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |