Monoclonal Anti-JAM2, (C-terminal) antibody produced in mouse
SIGMA/SAB1405233 - clone 1C2, purified immunoglobulin, buffered aqueous solution
Synonym: C21orf43; CD322; JAM-B; JAMB; PRO245; VE-JAM; VEJAM
Product Type: Chemical
Western Blotting Western Blot analysis of JAM2 expression in transfected 293T cell line by JAM2 monoclonal antibody, clone 1C2. Lanes Lane 1: JAM2 transfected lysate (33.2 kDa). Lane 2: Non-transfected lysate.
Western Blotting QC Western Blot detection against Immunogen (55.44 kDa).
ELISA Detection limit for recombinant GST tagged JAM2 is 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 1C2, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1κ |
| mol wt | antigen ~55.81 kDa |
| NCBI accession no. | BC017779   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P57087 |
| General description: | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs. (provided by RefSeq) |
| Immunogen: | JAM2 (AAH17779, 29 a.a. ~ 298 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence FSAPKDQQVVTAVEYQEAILACKTP |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |