Monoclonal Anti-PTPNS1, (C-terminal) antibody produced in mouse
SIGMA/SAB1405361 - clone 4C7, purified immunoglobulin, buffered aqueous solution
Synonym: BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS-1; SHPS1; SIRP
Product Type: Chemical
Immunofluorescence Immunofluorescence of monoclonal antibody to SIRPA on NIH/3T3 cell. [antibody concentration 10 μg/mL]
Western Blotting SIRPA monoclonal antibody, clone 4C7 Western Blot analysis of SIRPA expression in NIH/3T3.
Western Blotting SIRPA monoclonal antibody, clone 4C7. Western Blot analysis of SIRPA expression in Raw 264.7.
Western Blotting QC Western Blot detection against Immunogen (78.54 kDa).
ELISA Detection limit for recombinant GST tagged SIRPA is approximately 0.1 ng/mL as a capture antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 4C7, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2aκ |
| mol wt | antigen ~78.91 kDa |
| NCBI accession no. | BC026692   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | capture ELISA: suitable |
| indirect ELISA: suitable | |
| indirect immunofluorescence: suitable | |
| western blot: 1-5 μg/mL | |
| UniProt accession no. | P78324 |
| General description: | The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. (provided by RefSeq) |
| Immunogen: | SIRPA (AAH26692, 28 a.a. ~ 507 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Sequence VAGEEELQVIQPDKSVSVAAGESAI |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |