Anti-CRK antibody produced in mouse
SIGMA/SAB1405658 - purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between FLT1 and CRK. Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:600 and anti-CRK mouse purified polyclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting Western Blot analysis of CRK expression in transfected 293T cell line by CRK polyclonal antibody. Lanes Lane 1: CRK transfected lysate (22.90 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~22.9 kDa |
| NCBI accession no. | NM_005206   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | proximity ligation assay: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P46108 |
| General description: | This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. (provided by RefSeq) |
| Immunogen: | CRK (NP_005197.3, 1 a.a. ~ 204 a.a) full-length human protein. Sequence MAGNFDSEERSSWYWGRLSRQEAVA |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |