Anti-DDX5 antibody produced in mouse
SIGMA/SAB1405708 - purified immunoglobulin, buffered aqueous solution
Synonym: DKFZp434E109; DKFZp686J01190; G17P1; HLR1; HUMP68; p68
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to DDX5 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting DDX5 polyclonal antibody. Western Blot analysis of DDX5 expression in HeLa S3 NE.
Western Blotting DDX5 polyclonal antibody. Western Blot analysis of DDX5 expression in Raw 264.7.
Western Blotting Western Blot analysis of DDX5 expression in transfected 293T cell line by DDX5 polyclonal antibody. Lanes Lane 1: DDX5 transfected lysate (67.54 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~67.54 kDa |
| NCBI accession no. | BC016027   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P17844 |
| Application: | Anti-DDX5 antibody produced in mouse is suitable for indirect immunofluorescence and indirect ELISA. |
| Biochem/physiol Actions: | DDX5 (DEAD, Asp-Glu-Ala-Asp, box helicase 5) is associated with the regulation of pre-mRNA splicing mechanism. It acts as a transcriptional coactivator to control alternative splicing during the formation of spliceosome. DDX5 provides stability to the RNA secondary structure during several activities such as in the translation initiation, nuclear and mitochondrial splicing. It facilitates the unwinding as well as detachment of protein part from RNA. The protein also participates in the estrogen- and androgen-signaling pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. (provided by RefSeq) |
| Immunogen: | DDX5 (AAH16027, 1 a.a. ~ 614 a.a) full-length human protein. Sequence MSGYSSDRDRGRDRGFGAPRFGGSR |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |