Anti-DKC1 antibody produced in mouse
SIGMA/SAB1405716 - purified immunoglobulin, buffered aqueous solution
Synonym: CBF5; DKC; FLJ97620; NAP57; NOLA4; XAP101
Product Type: Chemical
Immunohistochemistry Immunoperoxidase of antibody to DKC1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 μg/mL]
Immunofluorescence Immunofluorescence of antibody to DKC1 on HeLa cell. [antibody concentration 10 μg/mL]
Western Blotting DKC1 polyclonal antibody. Western Blot analysis of DKC1 expression in A-431.
Western Blotting Western Blot analysis of DKC1 expression in transfected 293T cell line by DKC1 polyclonal antibody. Lanes Lane 1: DKC1 transfected lysate (56.54 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~57.7 kDa |
| NCBI accession no. | NM_001363   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1 μg/mL | |
| UniProt accession no. | O60832 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene. Mutations in this gene cause X-linked dyskeratosis congenita, a disease resulting in reticulate skin pigmentation, mucosal leukoplakia, nail dystrophy, and progressive bone marrow failure in most cases. Mutations in this gene also cause Hoyeraal-Hreidarsson syndrome, which is a more severe form of dyskeratosis congenita. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq) |
| Immunogen: | DKC1 (NP_001354, 1 a.a. ~ 514 a.a) full-length human protein. Sequence MADAEVIILPKKHKKKKERKSLPEE |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |