Anti-FN1 antibody produced in mouse
SIGMA/SAB1405825 - purified immunoglobulin, buffered aqueous solution
Synonym: CIG; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED-B; FINC; FN; FNZ
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to FN1 on HeLa cell. [antibody concentration 10 μg/mL]
Proximity Ligation Assay Proximity Ligation Analysis of protein-protein interactions between HGF and FN1. HeLa cells were stained with anti-HGF rabbit purified polyclonal 1:1200 and anti-FN1 mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blotting FN1 polyclonal antibody. Western Blot analysis of FN1 expression in human kidney.
Western Blotting Western Blot analysis of FN1 expression in transfected 293T cell line by FN1 polyclonal antibody. Lanes Lane 1: FN1 transfected lysate (23.21 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~17.93 kDa |
| NCBI accession no. | BC005858   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| proximity ligation assay: suitable | |
| western blot: 1 μg/mL | |
| UniProt accession no. | P02751 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. (provided by RefSeq) |
| Immunogen: | FN1 (AAH05858, 1 a.a. ~ 163 a.a) full-length human protein. Sequence MSESGFKLLCQCLGFGSGHFRCDSS |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |