Anti-HIF1A antibody produced in mouse
SIGMA/SAB1405933 - purified immunoglobulin, buffered aqueous solution
Synonym: HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78
Product Type: Chemical
Immunofluorescence Immunofluorescence of antibody to HIF1A on HepG2 cell. [antibody concentration 10 μg/mL]
Western Blotting Western Blot analysis of HIF1A expression in transfected 293T cell line by HIF1A polyclonal antibody. Lanes Lane 1: HIF1A transfected lysate (80.85 kDa). Lane 2: Non-transfected lysate.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~82.7 kDa |
| NCBI accession no. | NM_181054   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | Q16665 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified. (provided by RefSeq) |
| Immunogen: | HIF1A (NP_851397.1, 1 a.a. ~ 735 a.a) full-length human protein. Sequence MEGAGGANDKKKISSERRKEKSRDA |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |