Anti-HLA-A antibody produced in mouse
SIGMA/SAB1405936 - purified immunoglobulin, buffered aqueous solution
Product Type: Chemical
Western Blotting HLA-A polyclonal antibody. Western Blot analysis of HLA-A expression in A-431.
Western Blotting Western Blot analysis of HLA-A expression in transfected 293T cell line by HLA-A polyclonal antibody. Lanes Lane 1: HLA-A transfected lysate (40.15 kDa). Lane 2: Non-transfected lysate.
Western Blotting HLA-A polyclonal antibody. Western Blot analysis of HLA-A expression in human spleen.
Flow Cytometry FACS analysis of negative control 293 cells (Black) and HLA-A expressing 293 cells (Green) using HLA-A purified mouse polyclonal antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~40.8 kDa |
| NCBI accession no. | NM_002116   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: suitable |
| western blot: 1 μg/mL | |
| UniProt accession no. | P04439 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. (provided by RefSeq) The previously assigned protein identifier P01891 has been merged into P04439. Full details can be found on the UniProt database. |
| Immunogen: | HLA-A (NP_002107.3, 1 a.a. ~ 365 a.a) full-length human protein. Sequence MAVMAPRTLLLLLSGALALTQTWAG |
| Physical form: | Solution in phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |